Signalling
Part:BBa_K750115
Designed by: Sifan Wang,Yizhen Yan,Ruosang Qiu,Youbin Mo Group: iGEM12_XMU-China (2012-09-22)
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)
This part contains pBADcIT(BBa_K750104),PtetcIT(BBa_K750102),PcIGLT(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from PcIGLT. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1083
Illegal NheI site found at 1106 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3686
[edit]
Categories
Parameters
control | K206000 R0040 R0051 |
device_type | signalling |
proteins | cI GFP |